Integrated Kab rov tav Electrophoresis System

 

SVL-2 integrated kab rov tav electrophoresis ntsuas yog tsuas yog siv rau ceev agarose electrophoresis sib cais thwmsim ntawm me me ntawm DNA thiab RNA kuaj.

 

1. Lub gel txiag pwm yog integrally tsim, uas tuaj yeem ua plaub qhov sib txawv ntawm cov gel blocks.

2. Tus qauv xiav lub teeb transilluminator, nrog SPW-6S electrophoresis fais fab mov, tuaj yeem saib ncaj qha rau lub band thaum lub sij hawm electrophoresis txheej txheem.

3. Cov pob tshab sab saud muaj lub qhov tsim, uas yooj yim rau kev tshem tawm cov cua sov thiab kev soj ntsuam.

4. Daim npav qhov txwv muaj nuj nqi, kev ua haujlwm raug.

5. Lub taub hau electrode thiab lub taub hau electrode yog detachable, uas yog yooj yim rau tu thiab tu.

 

 

1	Cov khoom naj npawb	SVL-2
2	Specifications thiab Dimensions (LXWXH)	310 X 150 X 120 mm
3	Gel Tray Specifications (LXW)	Loj 120mm X 120mm; Widthgel 60mmX120mm; Ntev gel 120mm X 60mm; Smallgel 60mmX60mm
4	Specimen Comb Specifications	1.0 hli 25/11 hniav 1.5mm 13/6 hniav 2.0mm 3/2 tooth
5	Lub teeb xiav wavelength	470nm ua
6	Tsis muaj peev xwm	260 ml ib
7	Qhov hnyav	1kg ua

 

Thov xyuas kom meej tias kab rov tav electrophoresis tank, gel tais, qauv zuag, gel txiag thiab lwm yam khoom yog huv si thiab qhuav ua ntej gel npaj.

1. Kev npaj

Ntsuas tus nqi tsim nyog ntawm agarose rau hauv lub hwj Erlenmeyer, ntxiv qhov tsim nyog ntawm qhov tsis zoo, muab tso rau hauv dej da dej, lub tshuab cua sov sib nqus los yog lub qhov cub microwave thiab cua sov kom txog thaum nws yaj tag, co zoo thiab ua cov tshuaj agarose gel.

 

2. Gel Ua

Muab cov gel tsim kab rov tav rau ntawm lub rooj zaum sim, xaiv ib lub tais uas haum (qhov ntau thiab tsawg ntawm cov tais gel raug xaiv raws li qhov kev xav tau sib txawv, qhov tshwj xeeb ntawm cov tais gel yog 120mmX120mm, 120mmX60mm, 60mmX120mm, 0mm) 60mmX nws nyob rau hauv thegel txiag (ob daim ntawm 60mmX60mm gel tuaj yeem ua rau tib lub sijhawm, thiab tsuas yog ib daim tuaj yeem ua rau lwm yam tshwj xeeb) thiab muab cov qauv zuag rau hauv qhov chaw ruaj khov. Ntxiv 5ul SerRed (los yog lwm yam nucleic acid dyes) tov rau agarose gel txias kom txog 55 degree. Tom qab sib tov, ua tib zoo ncuav nws mus rau hauv lub tais gel, thiab maj mam kis cov gel kom txog rau thaum tag nrho cov saum npoo ntawm lub gel tais yog uniform. Rau cov gel txheej, cia sawv ntsug ntawm chav tsev kub kom txog thaum lub gel yog tag nrho solidified, thiab maj mam rub tawm cov qauv zuag vertically kom tiav cov kev npaj ntawm cov gel phaj.

 

3. Qauv Loading

Nqa cov gel npaj thiab cov tais gel tawm ntawm lub gel txiag ua ke thiab muab tso rau hauv lub thawv electrophoresis nrog cov qauv ntxiv qhov ze rau qhov tsis zoo electrode (dub yog qhov tsis zoo electrode). Ntxiv cov tsis khiav kom txog thaum lub gel phaj npog.

Siv 10ul micropipette ntxiv cov qauv rau cov qauv grooves ntawm cov roj hmab phaj feem. Tom qab txhua tus qauv ntxiv, yuav tsum tau hloov lub taub hau ntxiv kom tsis txhob muaj kab mob. Thaum ntxiv cov qauv, tsis txhob ua kom cov gel nto nyob ib ncig ntawm cov qauv qhov dej (ua tib zoo saib xyuas qhov kev txiav txim ntawm kev ntxiv cov qauv).

 

4. Electrophoresis

Npog lub hau saum toj kawg nkaus raws li txoj haujlwm raug ntawm tus ncej zoo thiab qhov tsis zoo (liab yog qhov zoo, dub yog qhov tsis zoo), ntxig cov hlau electrophoresis rau hauv cov khoom siv hluav taws xob electrophoresis raws li cov xim raug, thiab xaiv qhov tsim nyog voltage pib electrophoresis (lub tshwj xeeb electrophoresis tsis tau hloov kho raws li qhov tseeb sim tsis tau).

Cov qauv txav ntawm qhov tsis zoo (dub) mus rau qhov zoo (liab) kev taw qhia. Raws li qhov voltage nce, qhov kev sib cais zoo ntawm agarose gel txo qis. Lub electrophoresis tau nres thaum lub bromophenol xiav txav mus txog li 1 cm ntawm sab ntug ntawm lub gel phaj.

 

5. Kev soj ntsuam

Qhov ntsuas kab rov tav electrophoresis no tau nruab nrog lub teeb xiav lub teeb transilluminator, uas tuaj yeem soj ntsuam qhov xwm txheej electrophoresis hauv lub sijhawm tiag tiag nrog (SPW-6S electrophoresis fais fab mov ).

 

Serial tooj	Lub npe	Ntau
1	Kab rov tav electrophoresis apparatus	1set ib
2	Gel txiag	1
3	1.	4
4	1.5mm 13-tooth/6-cov qauv txhuam hniav	1
5	2.	1
6	60mm X 60mm Gel Tais	2
7	60mm X 120mm Gel Tais	1
8	120mmX60mm gel tais	1
9	120mmX 120mm gel tais	1
10	Blue lub teeb transilluminator	1set ib

 

1. Tsis txhob siv cov khoom no nrog hluav taws xob (lub npog sab saum toj ntawm kab rov tav electrophoresis apparatus yuav tsum tau them ua ntej lub zog electrophoresis qhib tom qab cov qauv ntxiv, thiab tsis txhob kov cov kab rov tav electrophoresis apparatus thaum lub sij hawm electrophoresis txheej txheem kom tsis txhob muaj kev phom sij. ntawm hluav taws xob shock).

 

2. Txhua qhov ntawm cov khoom no tuaj yeem ntxuav nrog dej, tsuas yog qhuav ib txwm los yog qhuav nrog daim ntawv nqus. Tsis txhob ci ntawm qhov kub thiab txias kom tsis txhob muaj kev puas tsuaj rau cov khoom.