Eosin Y Solution (Aqueous)

Eosin Y yog acidic dye siv nyob rau hauv histology rau staining cell plasma. Lub hauv paus ntsiab lus yog tias eosin dissociates rau hauv qhov tsis zoo them anions hauv dej, uas tuaj yeem khi rau cationic amino pawg ntawm cov proteins hauv cytoplasm thiab muab cov cytoplasm xim liab.

Qhov hnyav molecular ntawm Eosin Y siv hauv cov khoom no yog 649.89, thiab cov concentration yog 0.05%. Nws feem ntau yog siv nrog Hematoxylin staining tov (G1004) rau staining cov ntaub so ntswg los yog hlwb, thiab yog siv los qhia cov qauv ntawm tes. Tom qab staining, cell plasma qhia txawv ntxoov liab rau txiv duaj xim. Piv nrogG1001Eosin Y Solution (Alcoholic), cov ntaub so ntswg los yog hlwb stained los ntawm cov khoom no yuav tsum tau lub cev qhuav dej los ntawm gradient ethanol ua ntej xylene pob tshab thiab sealing, thiab cov xim ntawm cov ntaub so ntswg los yog cell plasma yog sib dua li ntawm G1001.

Khaws thiab thauj ntawm chav tsev kub. Siv tau rau 6 lub hlis.

Muab koj tus kheej xylene, gradient ethanol, anhydrous ethanol, thiab cov pos hniav nruab nrab.

1. Qauv ua ntej ua ntej

1) Rau paraffin seem: ntu tau ua ntu zus los ntawm xylene rau 10 min, hloov los ntawm xylene tshiab rau 10 min, anhydrous ethanol rau 5 min, tshiab anhydrous ethanol rau 5 min, 90% ethanol rau 5 min, 75% ethanol rau 5 min , thiab ntxuav hauv kais dej.

2) Rau cov seem khov: cov seem khov uas khaws cia ntawm -20 degree yuav tsum tau tso cai sawv ntsug rau 5-10 min kom rov qab mus rau chav sov.

2. Eosin Y stain

Cov seem kho saum toj no tau ncaj qha rau hauv Eosin Y Solution (Aqueous) rau 4-5 min, thiab tom qab ntawd ua kom lub cev qhuav dej los ntawm 70%, 80%, 95%, thiab 100% ethanol li ntawm 3 min txhua. Tom qab ntawd cov ntu tau qhuav dej los ntawm cov dej tsis huv ethanol tshiab rau 3 min, pob tshab hauv xylene rau 5 min, hloov los ntawm xylene tshiab thiab pob tshab dua rau 5 min, thiab thaum kawg kaw nrog cov pos hniav nruab nrab.

Lus Cim: Yog tias siv rau kev tuav pov hwm tom qab staining xws li immunohistochemistry, thov ua Eosin Y staining tom qab lwm qhov staining tiav.

1. Eosin Y tov (xws li cawv) tuaj yeem siv tau ntau zaus, txhua 100 mL ntawm Staining Solution tuaj yeem siv rau stains kwv yees li 150 ntu. Nws raug nquahu kom hloov cov xim tshiab thaum cov xim ntawm cov ntaub so ntswg pom tseeb tias ntiav.

2. Thov hnav lub tsho lab thiab cov hnab looj tes pov tseg thaum ua haujlwm.

Rau kev tshawb fawb siv nkaus xwb

Cim npe nrov: eosin y tov (aqueous), Tuam Tshoj eosin y tov (aqueous) manufacturers, lwm tus neeg, Hoobkas